国产亚洲高清在线精品不卡-国产亚洲精aa在线观看不卡-国产亚洲精彩视频-国产亚洲精品91-日本aⅴ日本高清视频影片www-日本aⅴ视频

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

簡(jiǎn)要描述:HTB-26 MDA-MB-231 人乳腺癌細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2525

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

ATCC® Number: HTB-26™ Price: $256.00

Designations: MDA-MB-231

Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: epidermal growth factor (EGF), expressed

transforming growth factor alpha (TGF alpha), expressed

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,13

D13S317: 13

D16S539: 12

D5S818: 12

D7S820: 8,9

THO1: 7,9.3

TPOX: 8,9

vWA: 15,18

Cytogenetic Analysis: The cell line is aneuploid female (modal number = 64, range = 52 to 68), with chromosome counts in the near-triploid range. Normal chromosomes N8 and N15 were absent. Eleven stable rearranged marker chromosomes are noted as well as unassignable chromosomes in addition to the majority of autosomes that are trisomic. Many of the marker chromosomes are identical to those shown in the karyotype reported by K.L. Satya-Prakash, et al.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1-2

PGM1, 1-2

PGM3, 1

Age: 51 years adult

Gender: female

Ethnicity: Caucasian

Comments: The cells express the WNT7B oncogene [PubMed: 8168088].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C without CO2.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

purified DNA:ATCC 45518

purified DNA:ATCC 45519

purified DNA:ATCC HTB-26D

purified RNA:ATCC HTB-26R

References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247

22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

22977: Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

23010: Dickstein B, et al. Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line. J. Cell. Physiol. 157: 110-118, 1993. PubMed: 8408230

23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088

26321: Satya-Prakash KL, et al. Cytogenetic analysis on eight human breast tumor cell lines: high frequencies of 1q, 11q and HeLa-like marker chromosomes. Cancer Genet. Cytogenet. 3: 61-73, 1981. PubMed: 7272986

32272: Katayose Y, et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res. 57: 5441-5445, 1997. PubMed: 9407946

32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

32341: Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194

32489: De Vincenzo R, et al. Antiproliferative activity of colchicine analogues on MDR-positive and MDR-negative human cancer cell lines. Anticancer Drug Des. 13: 19-33, 1998. PubMed: 9474240

33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443




















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 亚洲免费视频网址 | 男人j桶进女人p无遮挡 | 国内不卡一二三四区 | 激情影院网站 | 欧美日韩中文国产 | 成人毛片基地 | 欧美视频一区二区 | 第一福利影院 | 污视频免费看网站 | av毛片在线看 | 手机在线精品视频 | 亚洲一区二区三区首页 | 在线观看中文字幕一区 | 亚洲精品国产精品国自产网站 | 四虎国产在线 | 色婷婷精品 | 精品免费国产一区二区三区 | 欧美日韩一区二区综合 | 免费人成在线水蜜桃视频 | 久久久久国产精品 | 亚洲免费视频在线观看 | 欧美人成片免费看视频不卡 | 亚洲国产第一区二区三区 | 欧美精品 在线播放 | 国产精品免费一区二区三区 | 在线观看一区二区三区视频 | 久久99久久99精品免观看动漫 | 亚欧美视频 | 免费色视频在线观看 | 午夜国产大片免费观看 | 21天理论泰剧在线观看全集高清 | 中文字幕日本不卡一二三区 | 国产高清精品久久久久久久 | aaa在线观看 | 国内视频精品 | 手机看片日韩日韩韩 | 亚洲一级香蕉视频 | 国产一区二区在线免费观看 | 视频91在线| 免费人成网站在线高清 | 成人三级在线 |